Microtubules are filamentous structures in cells that maintain cell shape and facilitate the movement of molecules within the cell. They are long, hollow cylinders with a diameter of about 25 nm. It is possible to incorporate fluorescent molecules into microtubules; when illuminated by an ultraviolet light, the fluorescent molecules emit visible light that can be imaged by the optical system of a microscope. Required:
If the emitted light has a wavelength of 550nm and the NA of the microscope objective is 1.3, what is the smallest resolvable separation between two objects in the microscope?