What led to Japan’s isolation

The Tokugawa shared Hideyoshi's suspicions that Christian missionary work could be a pretext for a future invasion of Japan by one of the European powers. In 1635, shogun Tokugawa Iemitsu decided that the only way to ensure Japan's stability and independence was to cut off almost all contact with other nations

Explanation:

bacteria – a word that often brings out a sense of uneasiness in most of us are, in fact, one of the earliest forms of life. it can be quite unnerving to know that there are bacteria all over your skin, hair, and even inside the body. but only a few species of bacteria are dangerous. the majority of bacteria are good, and without them, life on earth wouldn’t be possible. let’s learn more about how are bacteria essential for you to survive.

citizens were able to charge more for their goods and services