Based on this map, how could the election of 1860 & President Abraham Lincoln have contributed to sectional differences between the North and the South prior to the civil war?

The specific department of health and human services (hhs) regulations that apply to research with children are known as: subpart b: additional protections for children involved as subjects in researchsubpart a: the common rulesubpart d: additional protections for children involved as subjects in researchsubpart c: protections for children in research