Social Studies, 19.03.2021 20:50 powella033
During the second part of the lab, students compare the activity of the same enzyme obtained from two different sources, a commercial source and a wheat germ extract. The way we do this is by tracking nitrophenol concentration increase over time. Time points are stated in the manual. Use the standard curve equation to obtain nitrophenol produced.
The purpose is to obtain the enzyme concentration in the wheat germ extract.
Compare the plots obtained for each of the samples (commercial and wheat germ enzyme; scatterplots with linear trendline; slope is Vmax). Use the definition of Kp, rate constant for the formation of the product, to solve for enzyme concentration in the wheat germ extract. (Both solutions have the same enzyme, concentration is not known for the wheat germ extract, but it is known for commercial product; see the manual for the value.)
Insert a) nitrophenol amounts, b) the plot(s) depicting nitrophenol over time and c) calculation and value for [Ewg].
Kp = Vmaxc / [Ec] = Vmaxwg / [Ewg]
Commercial acid phosphatase
Tube
A410
A1
0.071
A2
0.14
A3
0.199
A4
0.33
A5
0.413
A6
0.595
Wheat germ acid phosphatase
Tube
A410
B1
0.066
B2
0.223
B3
0.264
B4
0.36
B5
0.404
B6
0.552
The concentration of the commercial product is = 0.102
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