•why is gc typically limited to molecules that weigh less than 400 amu?

Gas chromatography (GC) is a method of separating mixed compounds. The separation will depend on each compound's retention time. Compounds with higher molecular weights will elute out slower than those with lower molecular weight. This is due to their difference in boiling points. The larger the structure, the higher the boiling point.

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and after water is usually warmed by the sun the water temperature raises and then evaporates .