Investigators wish to purify an enzyme—a serine protease—using affinity chromatography. they attach to the matrix of an affinity column an antibody molecule that binds specifically to a short sequence of amino acids located in the enzyme’s active site. when they apply a mixture of proteins to the column, the protease adheres to the column and the other proteins pass through. to extract their purified enzyme from the column, the investigators add a large excess of the peptide that the antibody recognizes. what should they expect to occur after this treatment?
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