PNGase F cleaves N-linked oligosaccharides and removes the entire glycan from the polypeptide chain by hydrolyzing the amine on the amino acid sidechain that connects the glycan to the polypeptide chain. Given the following polypeptide sequence that was treated with PNGase F, where are the site(s) for glycosylation (pick the appropriate Section)? HINT: Where does N-linked glycosylation occur and what would happen to the glycosylated residue when PNGase F hydrolyzes the bond between glycan and amino acid?
AEWQNITKGF SEDRLLIAGT DSSDLQQILS LLESNKDLLL

Section 1 Section2 Section 3 Section 4

a. Section 1
b. Section 2
c. Section 3
d. Section4