What part of the bacterial cell is most involved in gram staining and why?

The bacterial cell is most involved in gram staining is cell wall because it decides the color strength in the bacteria.

Gram Staining:

It is method of classifying bacteria on the basis of thickness of the cell wall of bacteria. This method includes 4 steps.

All Bacterial cells take up the crystal violet in the first step. On decolonization, gram negative bacteria lose the violet color because of thin peptidoglycan layer.

Therefore, the bacterial cell is most involved in gram staining is cell wall because it decides the color strength in the bacteria.

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Cell wall

Explanation:

The cell wall is the part of the bacterial cell is most involved in gram staining. This is because the two types of gram bacteria which are known as the gram positive and gram negative bacteria have different and unique cell wall constituents.

The gram positive bacteria have a cell wall composed of peptidoglycan while the gram negative bacteria have cell wall composed of a thin layer of peptidoglycan and an outer layer of lipopolysaccharide. They give different results when stained ; gram positive bacteria retains the crystal violet color of the reagent used while the gram negative bacteria gives a pale reddish color.