Explain, in your own words, how you would use the light microscope to view a wet mount of a protist, assuming the microscope is plugged in and that the light source is on. Please use at least the following 9 structures in your description and underline them as you use them: stage, stage clip(s), stage knob(s), eyepiece, scanning objective lens, low power objective lens, high power objective lens, coarse focus knob, and fine focus knob.

A light microscope is an instrument commonly used in the laboratory which makes use of light rays for viewing and enlarging smaller objects/organisms.

The wet protist is first of all put on the slide then the slide is placed on the Stage which is the flat podium where the slides containing the samples are put for viewing.

The Stage clips are then used to hold the slide containing the wet protist in proper place for a better viewing result.

The Coarse and fine focus knobs are used to adjust the stage by raising and lowering it .

The major difference is that the coarse focus knobs move more rapidly and is used with a low power objective lens while the fine focus knobs move less rapidly and are to be carefully controlled as it functions with a high power objective lens

The Eyepiece which is also referred to as the Ocular lens is then used to view the protist.

However, the view can then be adjusted with the Scanning objective lens which provides an image of the protist with the least magnification of 4X when compared to other lenses.

The low power objective lens and high power objective lens have magnifications of 10X and 40X respectively and can be used to view smaller structures of the protists which the naked eyes and scanning objective lens can't view.

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Please find the detailed explanation/description on how to use the light microscope to view a wet mount of a protist. The 9 structures mentioned in the question are in CAPS.

Explanation:

- Firstly, the slide containing the wet mount of the protist is prepared and placed at the centre of a lowered STAGE. The STAGE CLIPS over the sides of the slide are used to hold it in place.

- The revolving turret or nosepiece is turned so that the LOWEST POWER OBJECTIVE LENS is in position i.e. directly above the sample/slide. The SCANNING OBJECTIVE LENS, the lowest objective lens with power of 4x is used initially.

- The slide is viewed through the EYEPIECE, and the image is brought into focus using the STAGE KNOBS.

- The COARSE FOCUS KNOB is turned until the protist on the slide is brought into broad focus. The FINE FOCUS KNOB is then used to bring the image on the slide into sharp focus.

N.B: The OBJECTIVE LENS should not touch the cover slip on the slide during this process.

- When the protist on the slide is clear using lowest power objective lens, the revolver turret can be rotated to allow HIGHER POWER OBJECTIVE LENS.

- Due to higher magnification of the HIGH POWER OBJECTIVE LENS, the protist might need to be refocused using the FOCUS KNOBS.

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