How does the concentration of the substrate in an enzyme-controlled chemical reaction change over time?

The substrate concentration decreases rapidly until a saturation point is reached where there is no further change in substrate concentration.

Explanation:

One of the most important parameters in discussion of rate of enzyme catalysed reaction is the substrate concentration. The rate of reaction increases smoothly as the rate of reaction until a point is reached and the rate of reaction becomes independent of substrate concentration. This is the saturation point.

in the given question, the partial pressure is calculated by using the ideal gas equation, that is, pv = nrt

here, p is pressure, v is volume = 25 l, n is number of moles = 0.0104 moles, r is gas constant = -0.0821 l.atm / mol.k

t is temperature = 273 k

p = nrt / v

p = (0.0104 mol × 0.0821 l.atm / mol.k × 273 k) / 25 l

= 0.0093 atm

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