Imagine that you are developing a TLC plate, but you get distracted and forget to take the TLC plate out. Later, when you remove your TLC plate from the developing chamber, you notice that the solvent front has reached the top of the TLC plate. How will the Rf values from this TLC plate compare with those of a student who stopped developing their TLC plate before the solvent front reached the top of the plate

The full form of TLC is Thin layer chromatography which is used in separating non volatile mixtures.

Explanation:

In the context, the Rf values could not be read properly or determined properly and accurately if the solvent front flows to the top of the plate. If the solvent reaches the plate's top, then the Rf values reading will be different from the TLC plate where the solvent did not reach the plate's top.

If the solvent front reaches the top, then it is not possible to accurately determine draw the line and to determine how far the spot went. And thus it would be difficult to solve the ratio as the plate would keep advancing or developing and also the compound spot keeps rising. As a result, spots reaches higher or rises than the normal rise but the solvent front does not change, it remains same. Thus, the Rf value would be completely off.

- decrease the number of plants and animals living in the water.

3 1/4 %

explanation: