Chemistry, 16.12.2020 16:00 pinsonjon1129

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Why does DNA dissolve in water?

Why is salt added to solution of DNA and water?

How does ethanol help the precipitate form?

After a pellet is formed, is the DNA in the pellet or the supernate?

Answers: 2

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Answers

Answer from: khalaflaf2684

Explanation:

Because of this, DNA and RNA can easily dissolve in water. ... Adding ethanol helps the Na+ and PO3- ions come together, because ions travel easier in ethanol than water. The supernate is removed and new ethanol is added in the second washing. This removes any residual salt that remained on the pelleted DNA.

Answer from: hjamya17

DNA is polar due to its highly charged phosphate backbone. Its polarity makes it water-soluble (water is polar) according to the principle "like dissolves like". ... This fact makes water a very good solvent for charged compounds like salts.

Your DNA's sugar phosphate backbone is charged. By adding salt, we help neutralize the DNA charge and make the molecule less hydrophilic, meaning it becomes less soluble in water. The salt also helps to remove proteins that are bound to the DNA and to keep the proteins dissolved in the water.

It is well known that Ethanol has a lower dielectric constant than water, making it to promote ionic bond formations between the Na+ (from the salt) and the PO3- (from the DNA backbone), further, causing the DNA to precipitate.

Explanation:

Ideally DNA needs to be precipitated with pellet and should not remain in supernatant. DNA is acidic in nature and therefore, needs optimum salt concentration in the buffer to be pelleted from a solution. At very low salt concentration or without salt DNA would remain in supernatant.

Answer from: Quest

For cyclohexane compounds drawn in a chair conformation: -axial bonds are vertical -equatorial bonds are parallel to two bonds within the ring. thus, as i assume the chair conformation is drawn: -the x group is in an axial position, as are the five other vertical bonds. -three axial bonds are on the top face of the ring -the other three axial bonds are on the bottom face the position of the second substituent on each ring carbon is equatorial. gauche groups must be on an adjacent carbon such as carbon 6 and carbon 2, and also gauche groups are rotated by 60 degrees relative to each other. drawing the newman projections for c1–c2 and c1–c6 aids in visualizing the groups that are gauche to the y group on c1. click this link and watch some academic videos that may you more understand the topic

Answer from: Quest

The answer is c. 320 g

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Why does DNA dissolve in water...

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