When you perform a three-point test cross to determine the linkage between three genes, you found that distance between the three genes were 16 and 8 map units. Of 1000 progeny produced in the test cross, you observe 7 double recombinants. What is the interference? a. 0.547
b. 0.453
c. 0.16
d. 0.08
e. 0.0128

The correct option is b. 0.453

Explanation:

To calculate the coefficient of coincidence, CC, we must use the next formula:

CC= observed double recombinant frequency/expected double recombinant frequency

Note:  

• Observed double recombinant frequency=total number of observed double recombinant individuals/total number of individuals

• Expected double recombinant frequency: recombination frequency in region I x recombination frequency in region II.

CC= (7/1000)/0.16x0.08

CC= 0.007/0.0128

CC=0.5468

The coefficient of interference, I, is complementary with CC.

I = 1 - CC

I = 1 - 0.5468

I = 0.4531

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